Date &

Program Site

Batch

Venue and Time

Details of the Program

Day 1

I & II

8:00-9:00 am

Breakfast and Registration

24-07-22

S. P. Hiremath Hall

(Sunday)

9.00-10.00 am

Inauguration of the workshop and overview of the

workshop

10.00-10.30 am

Tea Break

Department

of Biochemistry Jnana Sahyadri, Kuvempu University

10.30-11.30am

Keynote address: High-value Phytochemicals: Biosynthesis and overproduction through molecular and metabolic engineeringtechniques

Dr. Dinesh A. NagegowdaPhD.

Prof. AcSIR and Senior Principal Scientist

CSIR-CIMAP Research Center, Bengaluru.Karnataka.

India – 560065

11:30-12:00Noon

Pre-workshop test

12.00 Noon

Dividing in to batches (Each batch 15 members)

Batch 1: Training @ Dept. of Biochemistry, Kuvempu

University, Shankaraghatta

Batch 2: Heads to Sahyadri Science College (SSC),

Shivamogga

12.00- 1.30 pm

Title of the talk: Approaches for the extraction, isolation

and characterization of bioactive compounds in plants

Resource Person: Dr. Vadlapudi Kumar, Ph.D

Professor, Department of Biochemistry.

Shiva gangothri, Davangere University, Tolahunase,

Davangere. Karnataka

1.30-2.00 pm

LUNCH BREAK

Batch

2.00-6.00 pm

·      Extraction of phytochemicals fromplants

·      Quantitative        analysis         of        phytochemicals: (carbohydrates,    proteins,    phenols    and    reducing

sugars).

I

Day 2

Batch

9.00-12.00 noon

Scientific lectures

25-07-22

I

Dept. of

Title of the talk: Exploring the therapeutic bioactive

(Monday)

Biochemistry,

molecules from medicinal plants.

Department of Biochemistry

Jnana Sahyadri,

Kuvempu University

Resource Persons:

1. Prof. S. E. Neelagund

Dept. of Biochemistry, Kuvempu University,

Kuvempu

University

Shankaraghatta

Experimental Session (continuation of Day 1)

12.00-1.00 pm

Experimental Session (continuation of Day 1)

·   In vitro antioxidant assays: DPPH/FRAPmethods.

1.00-2.00 pm

LUNCH BREAK

2.00-6.00 pm

Experimental Session (continuation of Day 2) Preparation of SDS-PAGE Gels and Running of Protein Sample.

In vitro antioxidant assays: DPPH/FRAP methods.

Day 3

Batch

9.00-11.00 noon

Scientific lectures

Title of the talk: Isolation, Purification and characterization of bioactive peptides/proteins from plants by conventional and recombinant DNA techniques.

Resource Person:

Prof. G. J. Sathisha

Dept. of Biochemistry, Kuvempu University

26-07-22

I

Dept. of

(Tuesday)

Biochemistry,

Kuvempu

University

Department of Biochemistry Jnana Sahyadri, Kuvempu

University

11.00-12.00 pm

Experimental Session: Extraction, salt/solvent fractionation of proteins from edible tubers, dialysis, lectin assay and column packing (ion exchange).

12.00-1.00 pm

Scientific lectures

Title of the talk: Recombinant expression and purification of antimicrobial peptides.

Resource Person:

Dr. P. Niranjana

Associate Professor, Chairman, Dept. of Biochemistry, Kuvempu University

1.00-2.00 pm

LUNCH BREAK

2.00-6.00 pm

Experimental Session (Continued): Purification of proteins by Ion exchange chromatography and FPLC

Batch

Venue & Time

Details of the Programme

2:30 pm - 4:00 pm

Basic cell culture procedures:

Day 4 Sunday 24^th July

Batch II

Cell Thawing, Seeding and Trypsinization

4:00 pm – 4:15 pm Tea Break

2022.

4:15 pm to 6:00 pm

Experimental session:

Animal cell culture: Cell count and cell viability assay, MTT assay.

Molecular

Biomedicine

Laboratory

Batch

Venue & Time

Details of the Programme

9:00 am – 9:45 am

Scientific lecture

Day5

Title of thetalk:       Plants as anticanceragents

Resource Persons: Dr. C. K. Ramesh

Professor.

Department of Biotechnology Sahyadri Science College

Monday 25^th July

9:45 am – 10:30 am

Scientific lecture

Title     of     the     talk:     In-vitro     production    of phytochemicals in plant cell suspension culture Resource Persons: Dr. PradeepaK

Assistant Professor. Department of Biotechnology

Sahyadri Science College Kuvempu University

2022

Molecular

Batch II

Biomedicine

Laboratory

10:30 am – 10:45 am Tea Break

10:45 am -1:00 pm

Experimental Session:

Animal cell culture: Preparation of the cell lysate and protein estimation.

1:00 pm – 2:00 pm Lunch break

2:00 pm - 4:30 pm

SDS-PAGE and Immunoblotting

MTT assay continued.

4:30 pm - 4:45 pm tea break

4:45 pm – 6:00 pm

Immunoblotting continue.

Batch

Time

Experiment

9:00 am -9:45 am

Scientific lectures

Title of the talk: Plant based dietary lectins: promising future phytomedicines for immunotherapetics

Resource Person:

Dr. Pramod S.N.

Associate Professor,

Chairman, Dept. of Food Technology, Davangere University

9:45 am- 10:30 am

Scientific lectures

Title of the talk: Targeting Tumor Angiogenesis: a perfect way to battle against cancer

Resource Person:

Dr. B.T. Prabhakar Associate Professor Department of Biotechnology Sahyadri Science College

Shivamogga.

Day6

Batch II

Monday 26^th July

2022

Molecular

Biomedicine

Laboratory

10:30 am – 11:30 am

Immunoblotting continue.

11:30 am -11:45 am Tea Break

11:45 am – 1:15 pm

MTT assay continuation

1:15 pm -2:00 pm Lunch Break

02:00 pm -3:30 pm

Apoptotic assays

3:30 pm -4:30 pm

In-Vivo CAM assay

4:30pm – 4:45 pm Tea break

4:45 pm to 6:30 pm

Demonstration of qRT-PCR.

Day 7

I & II

9.00-10.00 am

Scientific Sessions:

30-07-22

Title of the talk: Patenting of inventions based on

( Saturday)

traditional knowledge- Indian perspective.

Resource Person:

Dr. Sharangouda

Department of Biochemistry Jnana Sahyadri, Kuvempu University

Assistant Controller of Patents & Designs, The Patent office, Government of India.

GST road, Guindy, Chennai-600032, Tamil Nadu, India

10.00-11.00am

Scientific Session:

Title of the talk: Bioinformatics resources and tools for phytochemicals Resource Person:

Dr. Mahantesha B. N. Naika, PhD

Assistant Professor,

Dept. of Biotechnology & Crop Improvement,

Kittur Rani Channamma College of Horticulture, Arabhavi-

591218,

University of Horticultural Sciences, Bagalkot, Karnataka,

India.

11.00-11.15am

Tea Break

11:15-11:45am

Post Workshop Assessment Test

Prof. S. P.

Hiremath Hall,

Kuvempu

University

11:45-12:00noon

Participants feedback on the workshop

12.00-1.00 pm

Valedictory Function,

Prize Distribution

1:00-2:00 pm

LUNCH